Fast detection card for furazolidone metabolites-user manual
  The packaging testing methods for all 4 projects are the same:furazolin testing card(organization),furanetanone testing card(organization),furantoin testing card(organization),and furanetazone testing card(organization)
  This product is a qualitative detection used for residual detection of furazolidone metabolites in fish,shrimp,poultry,and other meats.The entire testing process only takes about 60 minutes and is suitable for on-site monitoring and various testing institutions.The detection limit of this product is 0.5~1ppb.
  [Detection Principle]
  The rapid detection card for pyrazolone metabolites applies the principle of competitive inhibition immunochromatography.During the flow process,pyrazolone metabolites in the sample bind to specific monoclonal antibodies labeled with colloidal gold,inhibiting the binding of antibodies and pyrazolone metabolite protein conjugates on the NC membrane detection line(T).If the content of furazolidone metabolites in the sample exceeds the detection limit,the color of the detection line(T)is lighter than that of the C line,and the result is positive;On the contrary,if the color of the detection line(T)is darker or the same as the quality control line(C),the result is negative
  Product composition:

Rapid detection card for furazolidone metabolites (20 pieces/box)	Extractant 5 (1 bottle/box, 8ml/bottle)
Extractant 1 (purified water) (1 bottle/box, 80ml/bottle)	Extractant 6 (one bottle/box, 80ml/bottle)
Extractant 2 (1 bottle/box, 10ml/bottle)	Extractant 7 (finished) (1 bottle/box, 10ml/bottle)
Extractant 3 (1 bottle/box, 10ml/bottle)	Extractant 8 (1 bottle/box, 10ml/bottle)
Extractant 4 (1 bottle/box, 20ml/bottle)	Instructions (1 copy/box)

  Instructions(1 copy/box)
  Self provided equipment and equipment are required:
  Stirrer,oscillator,electronic balance,centrifuge,air dryer,pipette;
  15mL centrifuge tube(for centrifugation),5mL centrifuge tube(for blow drying)
  Sample processing
  1.Take a certain amount of tissue sample,mix well with a stirrer,and weigh 2±0.1g of homogeneous material into a 15ml centrifuge tube.
  2.Then add 4ml of extractant 1,0.5ml of extractant 2,and 0.5ml of extractant 3 in sequence,vigorously shake for 3 minutes,and incubate at 60℃for 10-30 minutes.
  3.After incubation,add 1ml of extractant 4,0.4ml of extractant 5,and 4ml of extractant 6 in sequence,and vigorously shake for 3 minutes.
  4.Centrifuge at 4000r/min at room temperature(20-30℃)for 5 minutes,then transfer a certain amount of supernatant(see table below)into a 5ml centrifuge tube and dry with an air dryer at 65℃.
  The relationship between the amount of supernatant removed and the detection limit is as follows:
  Detection limit

detection limit	1ppb	0.5ppb
Transfer the volume of the supernatant	1.5ml	2.0ml

  2.0ml
  5.Add 0.5ml of extractant 7 to the dried centrifuge tube to dissolve the dried substance,then add 0.5ml of extractant 8 and vigorously shake for 30 seconds.Centrifuge at room temperature(20-30℃)at 4000r/min for 5 minutes,and transfer all the lower clear solution into a 1.5ml centrifuge tube for testing.
  [Usage Steps]
  1.Before conducting the test,please read the user manual in its entirety.Before use,restore the test card and sample solution to room temperature(20-30℃);
  2.Please use the testing card as soon as possible after removing it from the packaging bag;
  3.Place the detection card flat and take 80µl of the sample to be tested into the sampling well;
  4.Start timing when the liquid flows,react for 5 minutes,and determine the result based on the schematic diagram.Other time readings are invalid(when reading the result,place the detection card horizontally in front of the observer).
  【Result Interpretation】
  1.Negative(-):The color of the detection line(T)is darker than that of the quality control line(C),or it is the same depth,
  Indicates that the concentration of furazolidone metabolites in the sample is below the detection limit or does not contain any furazolidone metabolites.

  2.Positive(+):The detection line(T)shows a lighter color than the C line,or the detection line(T)does not show a color,indicating that the residual concentration of furazolidone metabolites in the sample is greater than the detection limit.
  3.Invalid:The quality control line(C)does not show color;It may be due to improper operation or failure of the detection card.A new detection card can be used for retesting.
  【Precautions】
  1.This pre-treatment can be used in conjunction with our company's rapid quality and safety testing box for aquatic products,without the need to purchase other instruments to complete an experiment.If you need relevant information,please contact our company.
  2.Disposable detection card;Do not use expired detection cards,and dispose of waste properly;Do not touch the white film surface in the center of the detection card;Do not reuse the equipped dropper to avoid cross contamination;Do not consume the reagents provided.
  3.When conducting sampling and testing operations,it is recommended to use live fish,live shrimp,or fish and shrimp that have been frozen for no more than two weeks as much as possible.Shrimp needs to undergo experiments after turning and shelling,while fish need to scale and skin themselves before taking parts of the fish's back that have less oil for experimentation.To ensure the accuracy of the experiment,it is advisable not to take parts of the fish's belly and tail that have more oil for experimentation,as more oil will adhere to the membrane surface of the observation area and affect the lateral chromatography system,interfering with the accuracy of the experimental results;
  4.If extractant 3 freezes,a slightly warm(30-40℃)water bath can be provided to accelerate its dissolution;
  5.Do not mix reagents and test cards from different batches.
  [Storage and expiration date]
  Store in a cool and dry place at 4-30℃,away from light;The validity period is 12 months(production date and batch number can be found in the packaging box)